万国企业网 » 新闻资讯 » » 小鼠可溶性转铁蛋白(STRF)elisa试剂盒

小鼠可溶性转铁蛋白(STRF)elisa试剂盒

放大字体  缩小字体 发布日期:2023-12-14  浏览次数:0
核心提示:检测抗原,定性定量分析,elisa试剂盒

小鼠可溶性转铁蛋白(STRF)elisa试剂盒贮藏条件:2~8℃低温保存;特异性:同时检测天然或重组的,且与其他相关蛋白无交叉反应;标本:血清/血浆/细胞上清液/尿液/体液/灌洗液/脑脊液/心房水/胸腹水/组织等;检测种属:人/大小鼠/鸡/犬/猴/兔/豚鼠/猪ELISA检测试剂盒等种属;研究域:炎症研究、生物学、研究、新陈代谢、信号通路、免疫学、传染病、其他研究;适用范围:此试剂盒仅用于科研实验,禁用于临床。上海轩泽康生物部分合作单位:中国农业大学;湖南大学;同济大学;华南理工大学;华南农业大学;华中科技大学协和深圳医院;宁夏回族自治区人民医院;交通大学仁济医院。

Assay procedure

1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.

2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.

3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.

4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C.

5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.

6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.

7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not

appear uniform, gently tap the plate to ensure thorough mixing.

8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.

66.jpg


 
关键词: 精细化学品,化学试剂,其他化学试剂
 
[ 加入收藏 ]  [ 告诉好友 ]  [ 打印本文 ]  [ 违规举报 ]  [ 关闭窗口 ]
 
  • 联系人:孙星
  • 地址:上海市金山区吕巷镇璜溪西街88号(吕巷经济园区)
  • 手机:13262730656