人谷氧还蛋白(GLRX) ELISA试剂盒结果计算: 1、 以标准品浓度做为横坐标(6 个标准品孔,加 1 个 0 值孔,共 7 个浓度点),对应的吸光 度(OD 值)作为纵坐标,利用计算机软件,采用四参数 Logistic 曲线拟合(4-pl),创 建标准曲线方程,通过样本的吸光度(OD 值),利用方程计算样品的浓度值。 2、 如果样品被稀释,通过上述方法测的浓度值,要乘以稀释倍数,才是样品的终浓度。
订购信息:
产品名称 | 规格 | 保存条件 | 检测方法 | 价格 |
人谷氧还蛋白(GLRX) ELISA试剂盒 | 96T/48T | 2-8℃条件中保存 | ELISA法 | 电议 |
人谷氧还蛋白(GLRX) ELISA试剂盒常见问题解答:
问:我的样本生成的值超出了分析的动态范围,我能用这些样本值么?
答:建议仅使用标准曲线范围内的样本值。标准曲线范围之外的值通常是非线性的,这可能会导致错误的外推值。产生高于高标准值的样品应(进一步)稀释并重复测定。如果样品低于测定范围,则认为样品不可检测。
Q:My samples generated values that were outside the dynamic range of the assay. Can I use these values?
A:It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted and the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable.