人滑行蛋白α(TXLNα) ELISA试剂盒常见问题解答:
1. 问:是否可以使用不同试剂盒的组分?答:每一个试剂盒包含具有特定批号的组件,以确保所有组件单独以及试剂盒中所有其他组件都能达到zui佳性能。对这些特定批次进行质量控制测试。绝不建议用户使用自己的组件或其他供应商试剂盒。Q:Can components from different kits be used?A:Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.
2. 问:我的标准曲线看起来很好,但我没有在样本中得到预期的信号,这是为什么?答:样品可能不含分析物。矩阵效应可能掩盖检测。确保按照我司试剂盒中说明书进行稀释。稀释,前请检查以确保稀释操作正确。过度稀释可能导致样品低于标准曲线的范围。
Q:My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?A:The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.
人滑行蛋白α(TXLNα) ELISA试剂盒组分与保存:
1) 仅供科研使用,不得用于临床诊断。
2)未开封的试剂盒保存在 2-8 度,不得使用过期试剂盒。
【组分 】、【数量 】、【主要成分】、【 开封后储存】
1.【校准品】、【 0.3ml/管】、【2-8℃14 天】;
2.【包被微孔板 】、【96T/48T】、【 预包被固相抗体】、【 2-8℃14 天 】;
3.【HRP 标记抗体】、【 10mL 】、【HRP 标记的检测抗体】、【 2-8℃180 天 】;
4.【样本稀释液 6mL】 、【 2-8℃180 天 】;
5.【底物液 A 】、【6mL】、【 0.01%过氧化氢】、【 2-8℃180 天 】;
6.【底物液 B】、【 6mL】、【 0.1%TMB】、【 2-8℃180 天】;
7.【终止液】、【 6mL】、【 2mol/L 稀硫酸】、【 2-8℃180 天】;
8.【20×浓缩洗涤液】、【 25mL】、【 0.05%Tween20】、【 2-8℃180 天 】;
9.【说明书】、【 1 份 】;
10.【自封袋】、【 1 个】;
11.【不干胶】、【 2 片 】。