人饥饿素O乙酰基转移酶(GOAT) ELISA试剂盒等酶联免疫分析试剂盒实验问题解析：
1.问：是否可以使用不同试剂盒的组分？答：每一个试剂盒包含具有特定批号的组件，以确保所有组件单独以及试剂盒中所有其他组件都能达到zui佳性能。对这些特定批次进行质量控制测试。绝不建议用户使用自己的组件或其他供应商试剂盒。Q:Can components from different kits be used?A:Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.
2. 问：我的标准曲线看起来很好，但我没有在样本中得到预期的信号，这是为什么？答：样品可能不含分析物。矩阵效应可能掩盖检测。确保按照我司试剂盒中说明书进行稀释。稀释，前请检查以确保稀释操作正确。过度稀释可能导致样品低于标准曲线的范围。Q:My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?A:The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.
人饥饿素O乙酰基转移酶(GOAT) ELISA试剂盒内容及其配置：
试剂盒成份（2-8℃保存） | 96孔配置 | 48孔配置 | 配置 |
微孔酶标板 | 12孔×8条 | 12孔×4条 | 即用型 |
标准品 | 0.3mL*6管 | 0.3mL*6管 | |
样本稀释液 | 6mL*1瓶 | 3mL*1瓶 | 即用型 |
检测抗体-HRP | 10mL*1瓶 | 5mL*1瓶 | |
20×洗涤缓冲液 | 25mL*1瓶 | 15mL*1瓶 | 按说明书进行稀释 |
底物A | |||
底物B | |||
终止液 | |||
封板膜 | 2张（96） | 2张（48） | |
说明书 | 1份 | 1份 | |
自封袋 | 1个 | 1个 |
上海轩泽康生物有限公司zhuan业供应人饥饿素O乙酰基转移酶(GOAT) ELISA试剂盒。因流程简单、实验结果准确、容易操作等特点收到广大科研工作者的信赖与支持。我司任意一款ELISA检测试剂盒均提供免费代测，从标本收集、保存、预试验、实验、数据分析等全实验过程提供完整的技术指导！ELISA检测试剂盒种属有大鼠、人、兔、猪、犬、植物、豚鼠、小鼠、牛等其他动物，了解更多详情请来电联系我司销售人员！