上海轩泽康生物人基质金属蛋白酶13(MMP13)ELISA试剂盒优势特点:1):适用范围广泛——适用于体液、组织匀浆,细胞培养上清液、尿液等各种类型的样本。2):可检测指标齐全——生长因子、炎症因子、脂肪因子、基质金属蛋白酶等等。3):的优化方案——回收利用率高、可靠性强。4):原装进口试剂——高效性、灵敏性、特异性。5):规范包被操作——吸附均匀、吸附性好、空白值低、空底透明度高。人基质金属蛋白酶13(MMP13)ELISA试剂盒组成:30 倍浓缩洗涤液( 20ml×1 瓶) 、终止液 (6ml×1 瓶)、酶标试剂( 6ml×1 瓶 )、 标准品(64μg/L)【 0.5ml×1 瓶】、 酶标包被板 (12 孔×8 条 )、标准品稀释液( 1.5ml×1 瓶)、 样品稀释液 (6ml×1 瓶 )、 说明书 1 份 、显色剂 A 液 (6ml×1 瓶)、封板膜 2 张、 显色剂 B 液( 6ml×1/瓶 )、 密封袋 1 个 。
人基质金属蛋白酶13(MMP13)ELISA试剂盒常见问题解答:
1.问:我的标准曲线看起来很好,但我没有在样本中得到预期的信号,这是为什么?答:样品可能不含分析物。矩阵效应可能掩盖检测。确保按照我司试剂盒中说明书进行稀释。稀释,前请检查以确保稀释操作正确。过度稀释可能导致样品低于标准曲线的范围。Q:My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?A:The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.
2.问:你建议我该如何洗板?答:如果您使用的是自动洗板机,我们建议定期检查校正,并在清洗前用洗板机缓冲液冲洗系统。手动洗板机也是如此。或者使用中继器或洗涤瓶。用户应小心确保所有内容物都被吸入,并在无棉纸上轻敲干燥。Q:How do you recommend I wash my plate?A:If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, and that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated and the plate tapped dry on lint-free paper.