人胶原凝集素(Collectin)ELISA试剂盒高背景的原因是什么？答：1）洗涤不当：检查洗涤缓冲罐的容量，确保按说明书操作进行洗涤步骤。2）被污染的仪器：使用前确保基材没有被金属离子或氧化剂污染。在使用底物溶液前确保单独保存额外的底物溶液。3）酶标板暴露于光：可能导致酶标板呈蓝色。4）错误的温育时间/温度：通常遵循标准的温育时间和温度。然而如果在标准稀释系列中没有观察到强梯度的情况下，所有的孔都是强烈且均匀的颜色，那么可能需要在颜色显影时观察基质反应，以便更快的停止反应。Q:What are the reasons for High Background?A:1) Improper Washing: Check volume of washing buffer reservoir and make sure all recommended washing steps are performed. 2) Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 3) Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate. 4) Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times and temperatures. However, if all wells are intensely and equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.
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