鸡内毒素(LPS)ELISA试剂盒常见问题解答：
问：如果我提取了样本，我是否仍需要遵循试剂盒中说明书给出的建议稀释度？答：提取程序后所需的样品稀释量将受到所用方案中纯化和浓度的影响。稀释量或浓度必须由zui终用户确定。Q:If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?A:The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification and concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.
2.问：我期望得到的分析物的预期浓度是多少？答：给定分析物的量不仅可能因物种而异，也可能因组织和细胞来源而异。这些信息的zui佳来源是通过互联网在多个科学数据库中轻松访问的文献。Q:What is the expected concentration of analyte that I should expect to find?A:The amount of a given analyte may vary not only from species-to-species, but also between tissue and cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.
鸡内毒素(LPS)ELISA试剂盒操作流程：先将试剂盒从冰箱内取出室温平衡20~30分钟后，根据实验要求取出所需板条，剩余板条用自封袋密封放回4℃。设置标准品孔和样本孔，在标准品孔各加不同浓度的标准品50ul，在样本孔中加入待测样本50ul（空白孔不加）。随后在标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100ul。用封板膜封住反应孔，37摄氏度的恒温箱温育60分钟。进行洗板（也可用洗板机洗板）：弃去液体，吸水纸上拍干，每孔加满洗涤液静置1分钟，再甩去液体，吸水纸上拍干。如此重复洗板5次。在每孔加入底物A、底物B各50ul，37℃避光孵育15分钟。每孔加入终止液50ul，15分钟内，在450nm波长处测定各孔的OD值。关于鸡内毒素(LPS)ELISA试剂盒订购流程：致电/MAIL由我司客服提供咨询→确定产品（名称、规格、数量）→签约付费（签订合同）→签收货物→售后服务。