上海轩泽康生物拥有自己的实验室（上海）和技术团队，公司提供全方位的售前、售中、售后技术支持，为您解决实验过程中遇到的问题。想要了解更多实验代做服务，请来电咨询!关于鸡胰岛素样生长因子1(IGF-1)ELISA试剂盒说明：
1．试剂盒保存：-20℃（较长时间不用时）；2-8℃（频繁使用时）。
2．浓缩洗涤液低温保存会有盐析出，稀释时可在水浴中加温助溶。
3．中、英文说明书可能会有不同之处，请以英文说明书为准。（详情请咨询我司客服）
实验结果记载：一切试验的初始材料均应存档；质控血清的来源及测定值并注明是不是在控；初始挂号表应记载试剂盒来源、批号；签上试验者名字和审核者的名字。
Assay procedure
1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
3. Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.
4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C.
5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not
appear uniform, gently tap the plate to ensure thorough mixing.
8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.
