猪细胞色素C(CytC)ELISA试剂盒常见问题解答：
问：我的光密度比试剂盒中附带的说明书里的光密度稍高（或更低），这是为什么？答：光密度受很多物理条件的影响，如时间和温度。我们建议您缩短或延长基质溶液的*终孵育时间以进行补偿。Q:My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?A:The optical density is affected by a number of physical conditions such as time and temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.问：我的样本生成的值超出了分析的动态范围，我能用这些样本值么？ 答：建议仅使用标准曲线范围内的样本值。标准曲线范围之外的值通常是非线性的，这可能会导致错误的外推值。产生高于*高标准值的样品应（进一步）稀释并重复测定。如果样品低于测定范围，则认为样品不可检测。Q:My samples generated values that were outside the dynamic range of the assay. Can I use these values?A:It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted and the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable.
ELISA试剂盒包含以下各组分：
1） 已包被抗原或抗体的固相载体（免疫吸附剂）
2） 酶标记的抗原或抗体（结合物）
3） 酶的底物
4） 阴性对照品和阳性对照品（定性测定中），参考标准品和控制血清（定量测定中）
5） 结合物及标本的稀释液
6） 洗涤液
7） 酶反应终止液
8） 产品使用手册
