上海轩泽康生物ELISA试剂盒适用血清、血浆、组织匀浆液、细胞培养上清液、尿液等等多种标本类型。同时可检测动物类型丰富：人、猴、大鼠、小鼠、兔、猪、犬、牛、绵羊、鸡等。可检测指标齐全：白介素，干扰素，血管紧张素，肺炎衣原体，趋化因子、生长因子基质金属蛋白酶、炎症因子、血管生成素等等指标。本试剂盒采用ELISA法可定性、定量检测抗原抗体，适用于血清、血浆及细胞培养上清标本，**于实验室研究。注：标准品浓度都不相同，请联系客服索取相应说明书。
鸡禽流感H7抗原(AIV-H7 Ag)ELISA试剂盒常见问题解答：
1.问：我期望得到的分析物的预期浓度是多少？答：给定分析物的量不仅可能因物种而异，也可能因组织和细胞来源而异。这些信息的**来源是通过互联网在多个科学数据库中轻松访问的文献。Q:What is the expected concentration of analyte that I should expect to find?A:The amount of a given analyte may vary not only from species-to-species, but also between tissue and cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.
2.问：我的光密度比试剂盒中附带的说明书里的光密度稍高（或更低），这是为什么？答：光密度受很多物理条件的影响，如时间和温度。我们建议您缩短或延长基质溶液的*终孵育时间以进行补偿。Q:My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?A:The optical density is affected by a number of physical conditions such as time and temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.
检测前准备工作：1.请提前20分钟从冰箱中取出试剂盒，平衡至室温。2. 从冰箱中取出的浓缩洗涤液可能有结晶，属于正常现象；放置室温，轻摇均匀，待结晶完全溶解后再配置洗涤液。可将20ml浓缩洗涤液用蒸馏水或去离子水稀释配置成400ml工作浓度的洗涤液，未用完的放回4℃3.20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份20×洗涤缓冲液加19份蒸馏水。